Our new Clear+ tissue clearing method is the sole strategy that delipidates samples with no transform in morphology and with negligible impact on structural integrity.
Megatome is actually a vibrating microtome intended to part a wide range of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With higher blade vibrating frequency and minimized blade deflection, Megatome allows substantial-throughput tissue sectioning with uniform area profile, as well as minimal tissue harm and data reduction.
Antibodies may perhaps acquire months to diffuse by only some millimeters of tissue, that has a steep labeling gradient from floor to Main.
Megatome is created for precision: the blade vibrates at a better frequency and bigger amplitude assortment than other microtomes, and contains a distinctive deflection Manage mechanism.
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Our preformulated EasyIndex Remedy raises and homogenize the refractive index of delipidated tissue samples, rendering them thoroughly clear. This enables light penetration into your sample and makes certain the acquisition of substantial-resolution, in-concentrate image facts.
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eFLASH is usually a quick tissue megatomi.com labeling technique that enables for uniform entire-organ staining in twenty rounds of labeling.
Absolutely delipidate complete mouse brains or comparably sized samples in only one day with SmartBatch+, or in a single 7 days with our passive clearing kit.
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Leverage the Crystal clear+ tissue clearing process, together with eFLASH and patented stochastic electrotransport technologies, to speedily apparent and label whole organs. Important highlights and functions incorporate:
Our novel Defend tissue preservation procedure sorts intramolecular bonds using polyfunctional, flexible epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.